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Our manuscript entitled “Activation of GTP Hydrolysis in mRNA-tRNA Translocation by Elongation Factor G” by Wen Li, Zheng Liu, Ravi Kiran Koripella, Robert Langlois, Suparna Sanyal, and Joachim Frank has been accepted by Science Advances.

In the article we describe two structures of the ribosome bound to the H91A mutant of EF-G in the presence of GTP, one in the rotated PRE, one in the unrotated POST state. From an analysis (by MDFF fitting of existing structures) of the reconstructed density maps at 4.2 and 3.6 Å resolution, we were able to draw conclusions on the mechanism of GTPase activation. It is of methodological interest that altogether 4 complexes could be retrieved from the same sample, by application of RELION classification, two with and two without EF-G bound.